Enter the sequence

# Example for substitution:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC
# Example for inserttion:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC
# Example for deletiion:
CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC
#The three examples above represent the input formats of three different editing types.The bases to be edited for each of the three editing types are all marked with a pair of parenthese().
#For base substitution,it is indicated by backslash "/",and the example is used to replace A with T.
#For base insertions are indicated by the plus sign "+",and the example indicates that ATT will be inserted at the parenthesis position
#Base deletions are indicated with a plus sign -. Examples indicate that GCTGGCGCGA will be deleted at the parenthesis position;

 

PAM   Maximum edit-to-nick distance     

Maximum PBS length   Maximum RTT length  

Minimum PBS length   Minimum RTT length  

NUMBER of optimal pegRNAS  Assembly  

MINIMUM NICK-TO-NICK DISTANCE  MAXIMUM NICK-TO-NICK DISTANCE 

MINIMUM DOWNSTREAM HOMOLOGY 

 

History

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Strand Spacer PAM PBS RTT EditToNickDistance sgRNASpacer sgRNAPAM NickToNickDistance Efficiency(%) Time
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCG GGCCTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 22.403526306152344 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCG TCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 22.310272216796875 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCG GCCTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 22.2636661529541 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCG CGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 22.164257049560547 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCGG GGCCTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 21.747652053833008 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCGG TCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 21.68872833251953 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCGG GCCTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 21.613393783569336 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCGG CGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 21.565906524658203 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCG CTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 20.951190948486328 20220514125822
+ GCGTCAAGCCGGGGGGGCGC TGG CCCCCCCGG CTCGCGCAATCAGCG 6 GTAGTAGTCGCAGAGTTGCC AGG 22 20.75212860107422 20220514125822